NEW EXPERIMENT WITH CORRECTIONS

Feb 09^th 2012

I made another experiment today with corrections from last week’s mistakes. Instead of making 2 master mixes for 32 tubes; Ms. Urban suggested we make 4 big master mixes, 1 big tube for every 4 smaller tubes. After a little discussion, we also decided to make the standard MgCl₂ 2 microliters instead of the original 1.5. Other changes remain the same as last week.

We also set up the tubes in gradients as A1, B2, C3, D4, E4, F5, G6, and H7. This way when they are in the Thermo cycler group ZAL 76 of the standard and the altered group (D4 and E4), will kind copy each other. They will get the same temperature so we can really see the difference in the other groups.  The temperature of the Thermo cycler remained QIA42-57, meaning that the temperature with decrease from right to left (indicated with arrows on the right). The experiment will look like this in terms of temperature control and standard vs. altered group:

REGION

PRIMER

1

2

3

4

5

6

7

8

9

10

NAD 6

NAD 6 F-NAD 6 R

A

ZAL 73

ZAL 73

ZAL 73

C-

B

ZAL 74

ZAL 74

ZAL 74

C

C

ZAL 75

ZAL 75

ZAL 75

C

D

ZAL 76

ZAL 76

ZAL 76

C

NAD 6

NAD 6 F-NAD6 R

E

ZAL 76

ZAL 76

ZAL 76

C

F

ZAL 75

ZAL 75

ZAL 75

C

G

ZAL 74

ZAL 74

ZAL 74

C

H

ZAL 73

ZAL 73

ZAL 73

C

Next week, I am going to set up gel for my experiment.