Feb 09th 2012
I made another experiment today
with corrections from last week’s mistakes. Instead of making 2 master mixes
for 32 tubes; Ms. Urban suggested we make 4 big master mixes, 1 big tube for
every 4 smaller tubes. After a little discussion, we also decided to make the
standard MgCl2 2 microliters instead of the original 1.5. Other
changes remain the same as last week.
We also set up the tubes in
gradients as A1, B2, C3, D4, E4, F5, G6, and H7. This way when they are in the
Thermo cycler group ZAL 76 of the standard and the altered group (D4 and E4),
will kind copy each other. They will get the same temperature so we can really
see the difference in the other groups.
The temperature of the Thermo cycler remained QIA42-57, meaning that the
temperature with decrease from right to left (indicated with arrows on the
right). The experiment will look like this in terms of temperature control and
standard vs. altered group:
REGION
|
PRIMER
|
1
|
2
|
3
|
4
|
5
|
6
|
7
|
8
|
9
|
10
|
|
NAD 6
|
NAD 6 F-NAD 6 R
|
A
|
ZAL 73
|
ZAL 73
|
ZAL 73
|
C-
|
 |
|||||
 |
B
|
ZAL 74
|
ZAL 74
|
ZAL 74
|
C
|
|||||||
C
|
ZAL 75
|
ZAL 75
|
ZAL 75
|
C
|
||||||||
D
|
ZAL 76
|
ZAL 76
|
ZAL 76
|
C
|
 |
|||||||
NAD 6
|
NAD 6 F-NAD6 R
|
E
|
ZAL
76
|
ZAL 76
|
ZAL 76
|
C
|
||||||
 |
F
|
ZAL 75
|
ZAL 75
|
ZAL 75
|
C
|
 |
||||||
G
|
ZAL 74
|
ZAL 74
|
ZAL 74
|
C
|
||||||||
H
|
ZAL 73
|
ZAL 73
|
ZAL 73
|
C
|
Next week, I am going to set up gel for my experiment.
