MAKING NEW NAD6- F, R AND PRESENTATION

April 04^th

Today we were going to make another PCR reaction, but we realized we didn’t have NAD6- F, R. But neither Mr. Adams nor I knew where the new bunch of NAD6 was located. Took us a while to find them so we looked for help to guide us through making some NAD6 from the concentrated bunch.

According to another researcher in the building we needed to make a mixture that we can use in an experiment with 9 part pure water and 1 part NAD6. If we put too little water the concentration wont bind the DNA, if the water is too much the solution will be diluted and we wont be able to work with it.

So to make a solution enough for the tubes, we needed 50 parts of NAD. So I made a mix of 45 part Water and 5 parts NAD6. So with this mixture next week we can make another PCR reaction put it under the same conditions in the Thermo cyclers and see how they work out.
Afterwards, Mr. Adams and I went over some ideas for my presentation, whether it be in a poster or during assembly. We went over some basic ideas that are crucial to mention in the presentation, the history of how the species were categorized before. We talked more about the morphology hypothesis and  examine the moths so that I know what their difference are physically.
Next week I am to bring a camera so that I can take some photos to include in my presentation. Its been a great semester at the internship, I have and am continuing to learn so much about science, research,lab work and my future plans to work in this field of science (lab/research).